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Originally published in Science Express on 13 November 2008
Science 12 December 2008:
Vol. 322. no. 5908, pp. 1710 - 1713
DOI: 10.1126/science.1165322
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Reports

A Role for the ESCRT System in Cell Division in Archaea

Rachel Y. Samson,1* Takayuki Obita,2 Stefan M. Freund,3 Roger L. Williams,2 Stephen D. Bell1*{dagger}

Archaea are prokaryotic organisms that lack endomembrane structures. However, a number of hyperthermophilic members of the Kingdom Crenarchaea, including members of the Sulfolobus genus, encode homologs of the eukaryotic endosomal sorting system components Vps4 and ESCRT-III (endosomal sorting complex required for transport–III). We found that Sulfolobus ESCRT-III and Vps4 homologs underwent regulation of their expression during the cell cycle. The proteins interacted and we established the structural basis of this interaction. Furthermore, these proteins specifically localized to the mid-cell during cell division. Overexpression of a catalytically inactive mutant Vps4 in Sulfolobus resulted in the accumulation of enlarged cells, indicative of failed cell division. Thus, the archaeal ESCRT system plays a key role in cell division.

1 Medical Research Council (MRC) Cancer Cell Unit, Hills Road, Cambridge CB2 0XZ, UK.
2 MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK.
3 MRC Centre for Protein Engineering, Hills Road, Cambridge CB2 0QH, UK.

* Present address: Sir William Dunn School of Pathology, South Parks Road, Oxford, OX1 3RE, UK.

{dagger} To whom correspondence should be addressed. E-mail: stephen.bell{at}path.ox.ac.uk

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The eocyte hypothesis and the origin of eukaryotic cells.
J. M. Archibald (2008)
PNAS 105, 20049-20050
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